Assistant Professor of Internal Medicine and
Adjunct Assistant Professor of Microbiology and Immunology
B.Sc. University of British Columbia
M.Sc. University of British Columbia
M.D. Wake Forest University School of Medicine
Daniel Leung's Lab Page
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Molecular Biology Program
MAIT cells, cholera, oral vaccines, diarrheal diseases
Mucosal associated invariant T (MAIT) cells in mucosal infections
MAIT cells are recently identified innate-like lymphocytes. They are primarily found in mucosal tissues and represent up to 10% of circulating T cells in humans. They are restricted by MR1, the non-classical MHC class I related protein, and MAIT ligands belong to a class of transitory intermediates of the riboflavin synthesis pathway. MAITs are capable of releasing both pro- and anti- inflammatory cytokines in response to stimulation, and possess cytotoxic activity
Cholera. We have recently reported that MAIT cells are activated in cholera and are associated with higher class-switched V. choleraepolysaccharide-specific antibody responses. In pilot studies, we have identified a subset of MAIT cells that express genes associated with B cell help. Additionally, in in vitro experiments, we show that MAIT cells can induce B cells to differentiate and produce antibodies. Thus, in collaboration with the International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), we are examining the role that MAIT cells play in the adaptive response against V. cholerae infection and vaccination. We are characterizing the clonal expansions of MAIT cells during human V. cholerae infection and oral cholera vaccination. At the same time, we are examining the mechanisms through which MAIT cells affect B cell differentiation and antibody production. Through these studies, we hope to gain new information on the capacity of MAIT cells to impact polysaccharide-specific antibody responses, which are associated with protection against cholera. This information has the potential to critically inform the development of better vaccine strategies targeted at preventing cholera and other enteric infections in young children.
Sepsis. Recent studies have found that septic patients have an early decrease in circulating MAITs, associated with an increased risk of secondary infections. We have found that MAITs from septic patients are highly dysfunctional, and that animals deficient in MAITs have a much higher mortality from experimental sepsis. Our goal is to determine how MAIT cells contribute to protective host responses by regulating inflammatory mediators. We are using a number of immunologic, genomic, and epigenetic techniques to examine the role of MAIT cells in both human and experimental models of sepsis.
Estimating Cholera Burden with Cross-sectional Immunologic Data
Identifying key populations at high risk of cholera is essential to guide global efforts to fight cholera, including targeting use of the oral cholera vaccine. Current methods to estimate cholera burden are largely based on clinical reporting with infrequent microbiological confirmation. These methods are limited by the sporadic nature of outbreaks, poor surveillance infrastructure, and fundamental uncertainties in the number of asymptomatic or mildly symptomatic cases. Detection of immune responses in serum (serosurveillance) can provide a new avenue for rapid and accurate estimates of cholera exposure and risk. We currently do not understand what immunological and clinical parameters are most predictive of recent exposure, nor whether immune responses in areas with different levels of endemicity are similar. We are pioneering the use of longitudinal antibody response kinetics, paired with novel statistical and machine learning approaches, to provide generalizable tools to estimate the incidence of exposure to Vibrio cholerae from cross sectional serosurveys. In collaboration with scientists at the icddr,b in Bangladesh, GHESKIO in Haiti, Johns Hopkins, and University of Florida, we are developing models to estimate the time since exposure to Vibrio cholerae and exposure incidence from cross-sectional antibody profiles and demographic data from cohorts of patients in Bangladesh and Haiti. We are also working to optimize and validate field-adapted methods to measure cholera-specific antibodies, including the use of dried blood spotand lateral flow assays. Our goal is to develop new tools to measure susceptibility to cholera in a population. These tools will have the potential to transform cholera control efforts from the current reactive strategies to proactive ones, with the potential to contribute to disease elimination.
Development of clinical decision tools for management of diarrhea of children in high and low resource settings
Diarrheal diseases are the among the leading cause of death in children worldwide, most of which occur in low-income countries. In high-income countries, pediatric diarrhea remains a major utilization of healthcare resources. Treatment of diarrhea is mostly empiric, with antibiotic use mostly based on clinical suspicion for bacterial causes. However, the majority of cases of diarrhea do not benefit from antibiotic use, and inappropriate use leads to toxicity and resistance. Furthermore, despite the increasing availability of rapid molecular testing, there is little data to base a decision of whom or when to test. Our goal is to develop and validate clinical decision tools for management of diarrheal illnesses in children of both high and low resource settings. In collaboration with investigators at Intermountain Healthcare and the University of Maryland, we are using machine learning and natural language processing methods to derive and validate such prediction tools. For a US-based prediction rule, we are using data from IMPACT, a study of 1200 children from 5 US Emergency Departments. For a prediction rule targeted at children in lower and middle-income country settings, we are using data from GEMS, a study of over 9400 children across 7 countries in sub-Saharan Africa and south Asia. Ultimately, we hope to make available of a number of clinical tools that healthcare workers worldwide can use for evidence-based care of children with diarrhea.
Respiratory immune dysregulation following intestinal infection
The human intestinal and respiratory tracts share a common mucosal immune system. Infections of the intestine and respiratory tracts are the two most common infections occurring in children in low-resource settings. Very little is known of how an intestinal infection may affect the immunity and health of the respiratory tract. We are examining how lung immune responses changes during an intestinal infection, and if susceptibility to pneumonia challenge is affected. Our goal is to determine whether (and how) intestinal infections affect the lung, and whether intervening on intestinal infections could help prevent respiratory health problems.
Our Research is funded by the following:
NIH/NIAID (R01AI130378, R01AI135114, R01AI135115)
DOD/CDMRP (PRMRP Discovery Award, W81XWH-17-1-0109)
University of Utah (III Initiative Seed Grant)
CDRF Global (US-Japan Joint Awards, OISE-17-62930-1)
Iyer AS, Azman AS, Bouhenia M, Deng LO, Anderson CP, Graves M, Kováč P, Xu P, Ryan ET, Harris JB, Sack DA, Luquero FJ, Leung DT. Dried Blood Spots for Measuring Vibrio cholerae-specific Immune Responses. PLoS Negl Trop Dis. 2018 Jan 29;12(1):e0006196.
Bliss J, Bouhenia M, Hale P, Couturier BA, Iyer AS, Rumunu J, Martin S, Wamala JF, Abubakar A, Sack DA, Luquero FJ, Couturier MR, Azman AS, Leung DT. High Prevalence of Shigella or Enteroinvasive Escherichia coli Carriage among Residents of an Internally Displaced Persons Camp in South Sudan. Am J Trop Med Hyg. 2018 Feb;98(2):595-597.
Andrews JR, Leung DT, Ahmed S, Malek MA, Ahmed D, Begum Y, Qadri F, Ahmed T, Faruque ASG, Nelson EJ (2017). Determinants of life-threatening diarrheal disease at hospital presentation: evidence from 22 years of admissions in Bangladesh. PLoS Negl Trop Diseases 11(4): e0005512.
Gruninger RJ, Johnson RA, Das SK, Nelson EJ, Spivak ES, Contreras JR, Faruque AS, Leung DT (2017). Socioeconomic Determinants of Ciprofloxacin-resistant Shigella Infections in Bangladeshi Children. Pathog Immun.
Michal Stevens A, Esposito DH, Stoney RJ, Hamer DH, Flores-Figueroa J, Bottieau E, Connor BA, Gkrania-Klotsas E, Goorhuis A, Hynes NA, Libman M, Lopez-Velez R, McCarthy AE, von Sonnenburg F, Schwartz E, van Genderen PJ, Scott Benson L, Leung DT (2017). Clostridium difficile infection in returning travellers.LID – 10.1093/jtm/taw099 [doi]. J Travel Med, 24(3).
Uddin MI, Islam S, Nishat NS, Hossain M, Rafique TA, Rashu R, Hoq MR, Zhang Y, Saha A, Harris JB, Calderwood SB, Bhuiyan TR, Ryan ET, Leung DT, Qadri F (2016). Biomarkers of Environmental Enteropathy are Positively Associated with Immune Responses to an Oral Cholera Vaccine in Bangladeshi Children. PLoS Negl Trop Dis, 10(11), e0005039.
Tsuha S, Taniguchi T, Shiiki S, Narita M, Leung DT (2016). Clinical characteristics of laboratory-confirmed leptospirosis in Okinawa, Japan, 1974-2015: high incidence of Jarisch-Herxheimer reaction. Trans R son Trop Med Hyg, 110(9), 558-565.
Iyer, AS, Bouhenia M, Rumunu J, Abubakar A, Gruninger RJ, Pita J, Lino RL, Deng LL, Wamala JF, Ryan ET, Martin S, Legros D, Lessler J, Sack DA, Luquero FJ, Leung DT, Azman AS (2016). Immune Responses to an Oral Cholera Vaccine in Internally Displaced Persons in South Sudan. Sci Rep, 6, 35742.
Leung DT, Bhuiyan TR, Nishat NS, Hoq MR, Aktar A, Rahman MA, Uddin T, Khan AI, Chowdhury F, Charles RC, Harris JB, Calderwood SB, Qadri F, Ryan ET (2014). Circulating Mucosal Associated Invariant T cells are Activated in Vibrio cholerae O1 Infection and Associated with Lipopolysaccharide Antibody Responses. PLoS Negl Trop Dis. 8(8):e3076.