Assistant Professor of Human Genetics
B.A. Cornell University
Ph.D. University of Michigan
Clement Chow's Lab Page
Clement Chow's PubMed Literature Search
Molecular Biology Program
Genetic variation, ER stress, Disease modifiers
Our lab is focused on understanding the role of genetic variation on disease outcomes. We employ quantitative and functional tools, in a variety of model organisms, to study how genetic variation impacts basic cellular traits important to human health. Our work in model organisms will help to model and inform studies of genetic variation in the human population. We hope to identify variation in the human population that can lead to more precise, personalized therapies.
Genetic variation is a powerful, unbiased tool to uncover novel aspects of gene networks. We are using natural variation to understand how endoplasmic reticulum (ER) stress varies among individuals in a population and what this means for disease variability. To do this, we are using the mouse Collaborative Cross to uncover the genetic architecture underlying this important cellular stress response. We are also utilizing Drosophila, mouse, and cell culture studies to uncover the function of genes that contribute to variability in the ER stress response.
In order to fully understand how genetic variation impacts the ER stress response, we are also undertaking studies to deeply characterize the genomic response to ER stress. How does the epigenome and transcriptional programming change when a tissue or cell is challenged with acute vs. prolonged ER stress? And more importantly, how do these states differ in a tissue- or cell-specific manner? To do this we are using RNAseq and various epigenetic tools to measure in vivo responses to ER stress in the mouse.
Natural genetic variation can also be used as a tool to identify novel modifiers of Mendelian diseases. It is widely appreciated that the expressivity of many Mendelian diseases can be quite variable. However, there is still a large gap in understanding why this variability exists. We use natural genetic variation in model organisms to study this variability in a controlled, tractable manner. We think that natural variation (rather than mutagenesis) in model organisms better models genetic variation in the human population. As a first step, we tested a well-studied retinitis pigmentosa mutation on ~200 strains of Drosophila and found extensive phenotypic variation in retinal degeneration. Using association study techniques we identified and validated numerous novel modifier genes. We aim to apply this paradigm to many Mendelian diseases to try to understand the nature of genetic modification.
- Chow CY. Bringing genetic background into focus. Nature Reviews Genetics. 17(2): 63-4 (2016).
- Chow CY, Kelsey K, Wolfner MF, Clark AG. Candidate genetic modifiers of retinitis pigmentosa
identified by exploiting natural variation in Drosophila. Hum. Mol. Gen. 25(4): 651-9 (2016).
- Chow CY, Avila F, Wolfner MF, Clark AG. Induction of excessive endoplasmic reticulum stress in the Drosophila male accessory gland results in infertility. PLoS ONE. 10(3): (2015).
- Chow CY, Wang X, Riccardi D, Wolfner MF, Clark AG. The genetic architecture of the genome-wide transcriptional response to ER stress in the mouse. PLoS Genet. 11(2): (2015).
- Chow CY, Wolfner MF, Clark AG. Using natural variation in Drosophila to discover previously unknown endoplasmic reticulum stress genes. PNAS. 110(22): 9013-9018 (2013).
- Chow CY, Wolfner MF, Clark AG. A large neurological component to genetic differences underlying biased sperm use in Drosophila. Genetics. 193(1): 177-8 (2013).
- Lenk GM, Ferguson CJ, Chow CY, Jin N, Jones JM, Grant AE, Zolov SN, Winters JJ, Giger RJ, Dowling JJ, Weisman LS, Meisler MH. Pathogenic mechanism of the FIG4 mutation responsible for Charcot-Marie-Tooth disease CMT4J. PLoS Genet. Jun 7(6) (2011).
- Im KM, Kirchhoff T, Wang X, Green T, Chow CY, et al. Haplotype Structure in Ashkenazi Jewish BRCA1 and BRCA2 Mutation Carriers. Hum Genet. 130(5): 685-99 (2011).
- Chow CY, Wolfner MF, Clark AG. The Genetic Basis for Male x Female Interactions Underlying Variation in Reproductive Phenotypes of Drosophila. Genetics. 186(4): 1355-65 (2010).
- Sirot LK, LaFlamme BA, Stitnik JL, Rubinstein CD, Avila FW, Chow CY, Wolfner, MF. Molecular social interactions: Drosophila melanogaster seminal fluid proteins as a case study. Adv. Genet. 68: 23-56 (2009).
- Chow CY, Landers JE, Bergren SK, Sapp PC, Grant AE, Jones JM, Everett L, Lenk GM, McKenna-Yasek DM, Weisman LS, Figlewicz D, Brown RH, Meisler MH. Deleterious Variants of FIG4, a Phosphoinositide Phosphatase, in Patients with ALS. Am J Hum Genet. 84(1): 85-8 (2009).
- Jin N*, Chow CY*, Liu L, Zolov SN, Bronson R, Davisson M, Petersen JL, Zhang Y, Park S, Duex JE, Goldowitz D, Meisler MH and Weisman LS. VAC14 nucleates a protein complex that is essential for the regulation of PI(3,5)P2 levels in yeast and mouse. EMBO J. (27), 3221-34 (2008). *co-first authors
- Zhang X, Chow CY, Sahenk Z, Shy M, Meisler M, Li J. Mutation of FIG4 causes a rapidly progressive, asymmetric neuronal degeneration. Brain 131, 1990-2001 (2008).
- McKinney B, Chow CY, Meisler M, Murphy G. Exaggerated emotional behavior in mice heterozygous null for the sodium channel Scn8a (Nav1.6). Genes, brain, and behavior 7 (6), 629-38 (2008).
- Zhang Y, Zolov S, Chow CY, Slutsky S, Richardson S, Piper R, Yang B, Nau J, Westrick R, Morrison S, Meisler M, Weisman L. Loss of Vac14, a regulator of the signaling lipid phosphatidylinositol 3,5-bisphosphate, results in neurodegeneration in mice. PNAS 104 (44), 17518-23 (2007).
- Chow CY, Zhang Y, Dowling J, Jin N, Adamska M, Shiga K, Szigeti K, Shy M, Li J, Zhang X, Lupski J, Weisman L, Meisler M. Mutation of FIG4 causes neurodegeneration in the pale tremor mouse and patients with CMT4J. Nature 448 (7149), 68-72 (2007).