Andrew Weyrich

Professor of Medicine and
Adjunct Professor in Pathology

Andy Weyrich

B.S. Baldwin-Wallace College

Ph.D. Bowman Gray School of Medicine



Andrew Weyrich's Lab Page

Andrew Weyrich's PubMed Literature Search


Our laboratory investigates molecular mechanisms that regulate cell/cell interactions, cell signaling, and gene expression in the inflammatory and hemostatic systems, and how these events become dysregulated in human diseases. The studies are broadly applicable to a variety of organ systems and disease processes, including atherosclerosis, diabetes, and sepsis. In previous work, we identified how key adhesion adhesion molecules on the surface of platelets induce gene expression pathways in target leukocytes. These studies also led to the unexpected discovery that platelets, which circulate without a nucleus, splice precursor mRNA and translate mature mRNA into protein.

Our group is currently using interrogation of cDNA libraries in combination with polyribosome analysis and other assays of specialized translational control, proteomic strategies, assays of mRNA splicing and stability, novel imaging methods, and cell biologic approaches to dissect pathways and consequences of expression of key genes in informative models (primary human leukocytes, platelets, endothelial cells, and human hematopoetic stem cells differentiated to specific mature cell phenotypes). In addition, our group co-directs a parallel group of projects that utilize genetically-altered mice to examine thrombosis and inflammation responses in vivo. The in vivo studies are performed in collaboration with Drs. Dean Li and Guy Zimmerman. In parallel studies at the bench, our group has ongoing investigations of cellular activities and phenotypes in inflammatory and thrombotic syndromes in human patients. Current projects include studies of novel platelet responses in sepsis and diabetes. A different group of studies involves infants with neonatal necrotizing enterocolitis and other neonatal inflammatory syndromes. The unifying feature of these disorders is dysregulated inflammation and hemostasis. Clinical fellows, post-doctoral fellows, graduate students, and junior faculty participate in each of these "bedside" studies, which serve as a basis for research career development awards. Thus, students and fellows in our group are exposed to parallel lines of investigation that employ modern biologic, genomic, and proteomic approaches using both in vitro and in vivo models. Discoveries using these tools are then extended to questions of human inflammatory, thrombotic, and pulmonary diseases in a translational fashion.


  1. Noetzli L, Lo RW, Lee-Sherick AB, Callaghan M, Noris P, Savoia A, Rajpurkar M, Jones K, Gowan K, Balduini CL, Pecci A, Gnan C, De Rocco D, Doubek M, Li L, Lu L, Leung R, Landolt-Marticorena C, Hunger S, Heller P, Gutierrez-Hartmann A, Xiayuan L, Pluthero FG, Rowley JW, Weyrich AS, Kahr WH, Porter CC, Di Paola J (2015) Germline mutations in ETV6 are associated with thrombocytopenia, red cell macrocytosis and predisposition to lymphoblastic leukemia. Nat Genet., 47:535-538
  2. Shi DS, Smith MC, Campbell RA, Zimmerman PW, Franks ZB, Kraemer BF, Machlus KR, Ling J, Kamba P, Schwertz H, Rowley JW, Miles RR, Liu ZJ, Sola-Visner M, Italiano JE Jr, Christensen H, Kahr WH, Li DY, Weyrich AS (2014) J Clin Invest., 124: 3757-3766
  3. Rondina MT, Brewster B, Grissom CK, Zimmerman GA, Kastendieck DH, Harris ES, Weyrich AS (2012) In Vivo Platelet Activation in Critically-Ill Patients with Primary H1N1 Influenza. Chest, 41(6):1490-5
  4. Kraemer BF, Campbell RA, Schwertz H, Franks ZG, Vieira de Abreu A, Grundler K, Kile BT, Dhakal BK, Rondina MT, Kahr WH, Mulvey MA, Blaylock RC, Zimmerman GA, Weyrich AS (2012) Blood 120: 5014-5020
  5. Kraemer BF, Campbell RA, Schwertz H, Cody MJ, Franks Z, Tolley ND, Kahr WH, Lindemann S, Seizer P, Yost CC, Zimmerman GA, Weyrich AS (2011) Novel anti-bacterial activities of ?-defensin 1 in human platelets: suppression of pathogen growth and signaling of neutrophil extracellular trap formation. PLoS Pathog, 7(11):e1002355
  6. Kahr WHA, Hinckley J, Li L, Schwertz H, Christensen H, Rowley JW, Pluthero FG, Urban D, Fabbro S, Nixon B, Gadzinski R, Storck M, Wang K, Ryu G-Y, Jobe SM, Schutte BC, Moseley J, Loughran NB, Parkinson J, Weyrich AS, Di Paola J (2011) Mutations in NBEAL2, encoding a BEACH protein, cause gray platelet syndrome. Nature Genetics, 43(8):738-40
  7. Rowley JW, Oler A, Tolley ND, Hunter B, Low EN, Nix DA, Yost CC, Zimmerman GA, Weyrich AS (2011) Genome wide RNA-seq analysis of human and mouse platelet transcriptomes. Blood, 118(14):e101-11
  8.  Schwertz H, Köster S, Kahr WH, Michetti N, Kraemer BF, Weitz DA, Blaylock RC, Kraiss LW, Greinacher A, Zimmerman GA, Weyrich AS (2010) Anucleate platelets generate progeny. Blood, May 6;115(18):3801-3809
  9. Foulks JM, Marathe GK, Michetti N, Stafforini DM, Zimmerman GA, McIntyre TM, Weyrich AS (2009) PAF-acetylhydrolase expressed during megakaryocyte differentiation inactivates PAF-like lipids. Blood 113:6699-706
  10. Weyrich AS, Denis MM, Schwertz H, Tolley ND, Foulks J, Spencer E, Kraiss LW, Albertine KH, McIntyre TM, Zimmerman GA (2007) Bcl-3 synthesis via mTOR controls the retraction of fibrin clots by activated human platelets. Blood, 109:1975-1983
  11. Denis MM, Tolley ND, Bunting M, Schwertz H, Jiang H, Lindemann S, Yost CC, Rubner FJ, Albertine KH, Swoboda KJ, Fratto CM, Tolley E, Kraiss LW, McIntyre TM, Zimmerman GA, Weyrich AS (2005) Escaping the nuclear confines: signal dependent pre-mRNA splicing in anucleate platelets. Cell, 122:379-391
  12. Galt SW, Lindemann S, Allen L, Medd DJ, Falk JM, McIntyre TM, Prescott SM, Kraiss LW, Zimmerman GA, Weyrich AS (2002) Outside-in signals delivered by MMP-1 regulate platelet function. Circ Res 90:1093-99
  13. Lindemann S, Tolley ND, Dixon DA, McIntyre TM, Prescott SM, Zimmerman GA, Weyrich AS (2001) Activated platelets mediate inflammatory signaling by regulated interleukin-1β Synthesis. J Cell Biol 154: 485-490
  14. Weyrich AS, Dixon DA, Pabla R, Elstad MR, McIntyre TM, Prescott SM, Zimmerman GA (1998) Signal-dependent translation of a regulatory protein, Bcl-3, in activated human platelets. PNAS 95:5556-5561

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