Distinguished Professor of Biochemistry
B.A. Swarthmore College
Ph.D. University of California, Berkeley
Dana Carroll's Lab Page
Dana Carroll's PubMed Literature Search
We work with the powerful, exciting and relatively new tools of precise genome engineering, using the ZFN, TALEN and CRISPR-Cas9 platforms. This technology, also called genome editing or gene editing, allows researchers to make specific, targeted changes at unique sites in the genomic DNA of essentially any cell or organism. To date the nuclease platforms have been used successfully to modify the genomes of more than 60 different organisms, from model species to crop plants to primates.
Much of our past work involved evaluating how nuclease-mediated genome modification works, with the goal of optimizing procedures and enhancing desired. Currently we are interested in other aspects of how the nucleases function and how the repair outcomes may be limited. In addition, we are collaborating with other groups on applications to human cells and potential clinical uses. We remain open to additional collaborations where our expertise and experience may be useful.
I am personally interested in the broader uses of genome editing and the societal implications of such uses. For example, this powerful technology offers many opportunities for improving food organisms, both crop plants and livestock. Medical applications of genome editing are in their infancy, but also have great promise. We want to ensure that such uses are developed safely and their benefits are distributed appropriately. Complex ethical and societal issues are raised by these prospects, particularly when modifications to the human germline are considered.
- Carroll, D., Van Eenennaam, A.L., Taylor, J.F., Seger, J. and Voytas, D.F. (2016) Regulate genome-edited products, not genome editing itself. Nature Biotechnology, 34: 477-479.
- Carroll, D. (2016) A perspective on the state of genome editing. Molecular Therapy, 24: 412-413.
- Carroll, D. (2014) Genome Engineering with Targetable Nucleases. Annu. Rev. Biochem. 83: 409-439. PMID: 24606144
- Cho, S.W., Lee, J., Carroll, D., Kim, J.-S. and Lee, J. (2013) Heritable gene knockout in C. elegans by direct injection of Cas9-sgRNA ribonucleoproteins. Genetics 195: 1177-1180.
- Beumer, K.J., Trautman, J.K., Christian, M., Dahlem, T.J., Lake, C.M., Hawley, R.S.,Grunwald, D.J., Voytas, D.F. and Carroll, D. (2013) Comparing ZFNs and TALENs for gene targeting in Drosophila. G3 3: 1717-1725.
- Beumer, K.J., Trautman, J.K., Mukherjee, K. and Carroll, D. (2013) Donor DNA utilization during gene targeting with zinc-finger nucleases. G3 3: 657-664.
- Bozas, A., Beumer, K.J., Trautman, J.K. and Carroll, D. (2009). Genetic analysis of zinc-finger nuclease-induced gene targeting in Drosophila. Genetics 182, 641-652.
- Beumer, K.J., Trautman, J.K., Bozas, A., Liu, J.-L., Rutter, J., Gall, J.G. and Carroll, D. (2008). Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases. Proc. Natl. Acad. Sci. USA 105: 19821-19826.