Janet E. Lindsley
Associate Professor of Biochemistry
B.S. Davidson College
Ph.D. University of Wisconsin, Madison
Janet Lindsley's Lab Page
Janet Lindsley's PubMed Literature Search
Research
Current Activities: Since closing my research laboratory in 2003, I have dedicated my efforts to teaching, curriculum design and educational research. My passion is teaching metabolism from an intuitive perspective, with a strong emphasis on nutrition. I am course director for Medical Biochemistry, Medical Nutrition and the graduate Regulation of Metabolism course. Since spring of 2007 I have led a group of dietitians, researchers and medical doctors in the development of a practical, mostly on-line Nutrition curriculum for medical students. My research has focused on the educational outcomes of this intervention on student performance and satisfaction.
Prior Research Program: From 1993 to 2003 my lab studied the mechanism of enzymes that alter chromosome structure. Our initial studies involved mostly steady-state and pre-steady-state kinetic analysis of DNA topoisomerase II (topo II). These studies were aimed at understanding how this enzyme utilizes the energy from ATP to transport one duplex of DNA through a transient break in another. We discovered that this homodimeric enzyme, with two identical ATP binding sites, acts asymmetrically with the two ATP hydrolyzed sequentially at different times during the reaction cycle.
These kinetic studies of topo II led us to two other projects. One of these was a genetic analysis of the involvement of topo II in the development of chromosome translocations. We developed a genetic selection for chromosome translocations using S. cerevisiae as our model organism. This selection system allowed us to analyze the involvement of DNA recombination, replication and repair proteins in chromosome translocations. The second project was a biochemical analysis of the complex of proteins that condense chromosomes. We purified and analyzed the condensin complex from yeast in an attempt to determine how it interacts with DNA and utilizes ATP in a mechanism that results in chromatin condensation.
References
1. Lindsley JE, Rutter J (2006) Whence Cometh The Allosterome? PNAS USA 103:10533-10535
2. Lindsley JE, Rutter J (2004) Nutrient sensing and metabolic decisions. Comp Biochem Physiol B Biochem Mol Biol. 2004 Dec;139(4):543-59
3. Stray JE, Lindsley JE (2003) Biochemical Analysis of the Yeast Condensin Smc2/4 complex: An ATPase that Promotes Knotting of Circular DNA. J. Biol. Chem. 278:26238-48
4. Tennyson RB, Ebran N, Herrera AE, Lindsley JE (2002) A Novel Selection System for Chromosome Translocations in Saccharomyces cerevisiae. Genetics 160:1363-1373
5. Baird CL, Gordon MS, Andrenyak DM, Marecek JF, Lindsley JE (2001) The ATPase Reaction Cycle of Yeast Topoisomerase II: Slow Rates of ATP Resynthesis and Pi Release. J. Biol. Chem. 276:27893-27898
6. Morris SK, Lindsley JE (1999) Novel Aspects of Topoisomerase II Inhibition by Etoposide Revealed by Kinetic Analysis. J. Biol. Chem. 274:30690-30696
7. Baird CL, Harkins TT, Morris SK, Lindsley JE (1999) Topoisomerase II Drives DNA Transport by Hydrolyzing One ATP. PNAS USA 96:13685-13690
8. Harkins TT, Lindsley JE (1998) Pre-steady State Analysis of ATP Hydrolysis by Saccharomyces cerevisiae DNA Topoisomerase II. 1. A DNA-dependent Burst in ATP Hydrolysis. Biochemistry 37:7292-7298
9. Harkins TT, Lewis TJ, Lindsley JE (1998) Pre-steady State Analysis of ATP hydrolysis by Saccharomyces cerevisiae DNA Topoisomerase II. 2. Kinetic Mechanism for the Sequential Hydrolysis of Two ATP. Biochemistry 37:7299-7312
